Specifications
Features
|
Specifications
|
Main Functions
|
Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution
|
Applications
|
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
|
Purification method
|
Mini spin column
|
Purification technology
|
Silica technology
|
Process method
|
Manual (centrifugation or vacuum)
|
Sample type
|
PCR products, enzymatic reaction solution
|
Sample amount
|
10-200μl
|
Recovery
|
≥80%
|
Elution volume
|
≥7μl
|
Time per run
|
≤10 minutes
|
Liquid carrying volume per column
|
800µl
|
Binding yield of column
|
10µg
|
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
-
High recovery efficiency - ≥80% DNA recovery
-
Fast - isolation can be completed in 10 minutes by column gel method
Kit Contents
Contents
|
D212002
|
D212003
|
Purification Times
|
100 Preps
|
250 Preps
|
Buffer DP
|
60 ml
|
120 ml
|
Buffer DW2
|
20 ml
|
50 ml
|
Elution Buffer
|
15 ml
|
30 ml
|
HiPure DNA Micro Columns
|
100
|
250
|
2 ml Collection Tubes
|
100
|
250
|
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.