| CAT NO | PRODUCT NAME | SIZE | PRICE |
|---|---|---|---|
| D214102 | HiPure DNA Clean Up Kit | 100 preps | $60.00 |
| D214103 | HiPure DNA Clean Up Kit | 250 preps | $150.00 |
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Crude DNA, enzymatic reaction solution |
|
Sample amount |
<150μl |
| Recovery | 80% |
| Elution volume | ≥15μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D214102 | D214103 |
| Purification Times | 100 Preps |
250 Preps |
| Buffer GWP | 30 ml |
60 ml |
| Buffer DW2 | 20 ml |
50 ml |
|
Elution Buffer |
30 ml |
60 ml |
|
HiPure DNA Mini Columns I |
50 |
250 |
| 2 ml Collection Tubes | 50 |
250 |
Storage and Stability
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
