Specifications
Features
|
Specifications
|
Main Functions
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Recover DNA fragments between 60bp-10kbp from agarose gel
|
Applications
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PCR, NGS, labeling, ligation and enzyme digestion, etc.
|
Purification method
|
Mini spin column
|
Purification technology
|
Silica technology
|
Process method
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Manual (centrifugation or vacuum)
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Sample type
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Agarose gel, PCR products, enzyme products
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Sample amount
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Agarose gel: ≤500mg
|
Recovery
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≥80%
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Elution volume
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≥15μl
|
Time per run
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≤20 minutes(1-24 samples)
|
Liquid carrying volume per column
|
800µl
|
Binding yield of column
|
35µg
|
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
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High recovery efficiency - ≥80% DNA recovery
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General - recover DNA from gel or enzyme-driven reaction solutions such as PCR
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Fast – isolation can be completed in 10-15 minutes by column gel method
Kit Contents
Contents
|
D211102
|
D211103
|
Purification Times
|
100 Preps
|
250 Preps
|
Buffer GDP
|
120 ml
|
250 ml
|
Buffer DW2
|
50 ml
|
2 x 50 ml
|
Elution Buffer
|
20 ml
|
30 ml
|
HiPure DNA Mini Columns II
|
100
|
250
|
2 ml Collection Tubes
|
100
|
250
|
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data