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D2111

CAT NO PRODUCT NAME SIZE PRICE
D211102 HiPure Gel DNA Mini Kit 100 preps $60.00
D211103 HiPure Gel DNA Mini Kit 250 preps $150.00
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Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Features Specifications
Main Functions Recover DNA fragments between 60bp-10kbp from agarose gel
Applications PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Agarose gel, PCR products, enzyme products
Sample amount
Agarose gel: 500mg
Recovery 80%
Elution volume 15μl
Time per run 20 minutes(1-24 samples
Liquid carrying volume per column 800µl
Binding yield of column 35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

  • High recovery efficiency - 80% DNA recovery
  • General - recover DNA from gel or enzyme-driven reaction solutions such as PCR
  • Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Contents D211102 D211103
Purification Times 100 Preps 250 Preps
Buffer GDP 120 ml 250 ml
Buffer DW2 50 ml 2 x 50 ml
Elution Buffer
20 ml
30 ml
HiPure DNA Mini Columns II
100
250
2 ml Collection Tubes 100 250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37 to dissolve.

Experiment Data


Purchase Guide
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  • HiPure Gel DNA Mini Kit
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