| CAT NO | PRODUCT NAME | SIZE | PRICE |
|---|---|---|---|
| 1291750 | MagPure Circulating DNA Rich Mini Kit | 50 preps | $199.00 |
| 12917200 | MagPure Circulating DNA Rich Mini Kit | 200 preps | $696.00 |
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Specifications
| Features | Specifications |
| Main Functions | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 0.6ml |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBind particles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface of MagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | 1292750 | 12927200 |
| Purification Times | 50 preps | 200 preps |
| MagPure Particles F |
1.2 ml |
4.5 ml |
| MagBind Particles |
1.2 ml |
4.5 ml |
| Proteinase K | 24 mg | 90 mg |
| Protease Dissolve Buffer |
1.8 ml |
10 ml |
|
Buffer MLB |
30 ml |
120 ml |
| Buffer GDP |
15 ml |
60 ml |
|
Buffer MAW1 |
40 ml |
250 ml |
|
Buffer MW2 |
20 ml |
50 ml |
|
Elution Buffer |
15 ml |
60 ml |
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
