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IVD5432

CAT NO PRODUCT NAME SIZE PRICE
IVD5432 MagPure Circulating DNA Mini Kit 200 preps $398.00
RELATED TAGS: NIPT/NIPPT
Introduction

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Specifications

Features Specifications
Main Functions Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications qPCR, NGS, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Serum, plasma
Sample amount 0.2-0.6ml
Elution volume ≥30μl
Time per run ≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - without labour

Kit Contents

Contents IVD5432
Purification Times 200
MagPure Particles G 14 ml
Carrier RNA 310 μg
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer MLK 250 ml
Buffer MAW1 250 ml
Buffer MW2* 2 x 50 ml
Elution Buffer 60 ml

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data


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