CAT NO | PRODUCT NAME | SIZE | PRICE |
---|---|---|---|
IVD4144 | HiPure FFPE RNA Kit | 50 preps | $298.00 |
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from FFPE tissue |
Applications | RT-PCR, cDNA synthesis, second generation sequencing |
Products |
RNA, miRNA |
Purification method | Mini spin column |
Purification technology | Silica technology, DNase |
Process method | Manual (centrifugation or vacuum) |
Sample type | FFPE slice, FFPE embedded tissue |
Sample amount |
Tissue: <6 mg FFPE: <6 slices |
Yield |
1-20μg |
Elution volume | ≥15μl |
Time per run | ≤30 minutes (after digestion) |
Liquid carrying volume per column |
800μl |
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA wasfinally eluted with low-salt buffer.
Advantages
Kit Contents
Contents | IVD4144 |
Purification Times | 50 Preps |
HiPure RNA Micro Columns | 50 |
2ml Collection Tubes | 50 |
Proteinase K |
24 mg |
Protease Dissolve Buffer |
1.8 ml |
DNase I |
600 μl |
DNase Booster Buffer |
1.5 ml |
Buffer DPS |
60 ml |
Buffer FRL |
15 ml |
Buffer RLC |
15 ml |
Buffer RWC* | 10 ml |
Buffer RW2* |
20 ml |
RNase Free Water |
10 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data