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R4143

CAT NO PRODUCT NAME SIZE PRICE
R414302 HiPure FFPE RNA Kit 50 preps $149.00
R414303 HiPure FFPE RNA Kit 250 preps $671.00
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Introduction

HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.

Details

Specifications

Features Specifications
Main Functions Isolation total RNA from FFPE tissue and section samples
Applications RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type FFPE tissue sample
Sample amount
6mg
Yield 20μg
Elution volume 20μl
Time per run 60 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg

Principle

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer.

Advantages

  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - several samples can be extracted in 60 minutes by column method
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be recovered at the level of PG

Kit Contents

Contents R414302 D414303
Purification Times 50 Preps 250 Preps
HiPure RNA Micro Columns
50
250
2ml Collection Tubes 50 250
Buffer DPS
60 ml
250 ml
Buffer FRL
15 ml
60 ml
Buffer RLC
15 ml
60 ml
Buffer RWC* 10 ml 50 ml
Buffer RW2*
20 ml
2 x 50 ml
Protease Dissolve Buffer
1.8 ml
10 ml
Proteinase K
24 mg
120 mg
RNase Free Water
10 ml
20 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

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  • HiPure FFPE RNA Kit
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