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  • D635102MagPure Plant DNA LQ Kit.png

D6351

CAT NO PRODUCT NAME SIZE PRICE
D635101 MagPure Plant DNA Kit 48 preps $119.00
D635102 MagPure Plant DNA Kit 96 preps $224.00
D635103 MagPure Plant DNA Kit 5 x 96 preps $995.00
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Introduction

MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from 50-100 mg plant, fungal tissue
Applications PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Conventional economic plant samples
Sample amount

Fresh / frozen plant samples: 100mg

Dried plant / seed samples:20mg

Elution volume ≥50μl
Time per run 60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • Safe - require no phenol or chloroform extraction
  • Fast - several samples can be extracted in 60 minutes
  • High purity - high quality DNA, completely remove inhibitors

Kit Contents

Contents D635101
D635102 D635103
Purification Times 48 Preps
96 Preps 5 x 96 Preps
MagPure Particles
1.7 ml
4 ml
18 ml
RNase Solution
0.6 ml
1.2 ml
6 ml
Buffer SPL 30 ml
60 ml 300 ml
Buffer PS 10 ml
20 ml 100 ml
Buffer GW1* 26 ml
53 ml 220 ml
Elution Buffer
10 ml
30 ml
120 ml

Storage and Stability

RNase Solution and MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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  • MagPure Plant DNA Kit
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