| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D316402 | HiPure SF Plant DNA Kit | 50 preps | $99.00 |
| D316403 | HiPure SF Plant DNA Kit | 250 preps | $447.00 |
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from100 mg simple plant |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount |
Fresh / frozen plant samples: ≤100mg gDried plant / seed samples: ≤20mg |
| Elution volume | ≥30μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316402 | D316403 |
| Purification Times | 50 Preps | 250 Preps |
|
RNase A |
10 mg |
50 mg |
|
Protease Dissolve Buffer |
1.8 ml |
5 ml |
| Buffer SPL | 30 ml | 150 ml |
| Buffer PS | 10 ml | 50 ml |
|
Buffer GW1 |
22 ml |
110 ml |
| Buffer GW2* | 12 ml | 2 x 50 ml |
|
Buffer AE |
15 ml |
60 ml |
|
HiPure gDNA Mini Columns |
50 |
2 x 125 |
|
2 ml Collection Tubes |
50 |
2 x 125 |
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
| Name | CAT NO |
Fresh / frozen tissue amount |
Dried tissue amount |
Column type |
Elution volume |
Yield (muscle) |
| D3187 | 150mg | 40mg | 1.5ml column | 50 - 100μl | 3-70μg | |
| D3163 | 5g | 1g | 50ml column | 0.7 - 3ml | 75-1250μg | |
| D3164 |
100mg |
20mg |
1.5ml column |
50 - 100μl |
3-50μg |
|
| D3167 |
50mg |
15mg |
96 well plate |
75 - 150μl |
3-30μg |
