Cell-Free DNA Blood Collection Tube (in short name Cell-Free DNA BCT) is a direct draw whole blood collection tube intended for collection, stabilization and transportation of cell-free plamsa or serum DNA and cellular genomic DNA present in nucleated cells. This productis for Research Use Only. Not for use in diagnostic procedures.

The implications of cfDNA in blood in clinical medicine were realized for about two decades. The cfDNA extracted from plasma or serum of cancer patients has shown characteristics typical of tumor DNA and may serve as noninvasive biomarkers for cancer  detection  and management.  It was also demonstrated the presence of fetal cfDNA in maternal plasma. Clinical  applications involving fetal cfDNA analysis include sex determination, single-gene disorders, aneuploidy detection and so on. Due to the low abundance of the cfDNA biomarkers of cancer or fetal origin, it is recommended that genomic DNA background levels be minimized to provide accurate measurements cfDNA levels.

The mixing cell protective reagent contained in Cell-Free DNA BCT can stabilize nucleated blood cells, preventing the release of cellular genomic DNA, inhibiting nuclease mediated degradation of cf-DNA, and contribute to the overall stabilization of cf-DNA. Blood samples collected in Cell-Free DNA BCT are stable for up to 14 days when stored at the recommended temperature range (6-37°C) or 72h when shipment.

Kit Contents

Storage and Stability

1.  When stored at 18-30°C, Cell-Free DNA BCT is stable through expiration date.

2. Blood samples collected in Cell-Free DNA BCT for cf-DNA analysis are stable for 14 days when stored between 6-37°C.

3.  Do not freeze unfilled Cell-Free DNA BCT.

4.  During extreme temperature conditions, Cell-Free DNA BCT needs proper insulation for shipment.