Specifications
Features
|
Specifications
|
Main Functions
|
Extract pathogen DNA/RNA from whole blood, body fluid, serum, plasma, sputum, immersion solution, tissue homogenate supernatant, etc.
|
Applications
|
RT-PCR,PCR,NGS
|
Products
|
Pathogen DNA and RNA
|
Purification method
|
Mini spin column
|
Purification technology
|
Silica technology
|
Process method
|
Manual (centrifugation or vacuum)
|
Sample type
|
Whole blood, plasma, serum, immersion solution, tissue homogenate supernatant
|
Sample amount
|
200μl
|
Elution volume
|
≥30μl
|
Time per run
|
30 mins
|
Liquid carrying volume per column
|
800μl
|
Binding yield of column
|
100μg
|
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA is finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
-
Fast - column purification, several samples can be finished in 30 mins
-
High quality - high purity total RNA/DNA can be directly used in various sensitive downstream applications
-
Safe - no phenol chloroform extraction required
-
Sensitive - DNA/RNA can be recovered at the level of PG
Kit Contents
Contents
|
IVD4179
|
Purification Times
|
50 Preps
|
HiPure Viral Mini Column
|
50
|
2ml Collection Tubes
|
100
|
2ml Bead Tubes
|
50
|
Protease K
|
50 mg
|
Protease Dissolve Buffer
|
5 ml
|
DNase I (Powder)
|
10 mg
|
DNase Buffer
|
6 ml
|
Buffer CLB
|
100 ml
|
Buffer SDS
|
5 ml
|
Reagent DX
|
1.5 ml
|
Buffer ACL
|
30 ml
|
Buffer VHB*
|
22 ml
|
Buffer RW2*
|
20 ml
|
Buffer AVE
|
15 ml
|
Storage and Stability
Proteinase K and DNase I (Powder) should be stored at 2–8°C upon arrival. However, short-term storage (DNase I up to 1 week, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.