R4181

CAT NO PRODUCT NAME SIZE PRICE
R418102 HiPure Bacterial RNA Kit 50 preps $209.00
R418103 HiPure Bacterial RNA Kit 250 preps $801.00
RELATED TAGS:
Introduction

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from bacteria culture
Applications RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Bacteria culture
Sample amount Bacteria: <10^9
Elution volume ≥30μl
Elution 30μl
Time per run ≤40 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.


Advantages

  • Fast - several samples can be extracted in 30 minutes
  • High purity - the purified RNA can be directly used in various downstream applications
  • High recovery - RNA can be recovered at the level of PG
  • Good repeatability - silica gel column purification technology can obtain ideal results every time
  • Broad spectrum - it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
  • Sufficient components - the kit contains carried lysozyme and glass beads


Kit Contents

Contents R418102 R418103
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 50 250
Glass Beads (0.1-0.2mm) 20 g 90 g
Lysozyme 90 mg 400 mg
Protease Dissolve Buffer 5 ml 15 ml
Buffer STE 10 ml 30 ml
MagZol Reagent 60 ml 270 ml
Buffer RW2* 20 ml 2 x 50 ml
RNase Free Water 10 ml 30 ml


Storage and Stability

MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide
Download
Related Products
Leave A Message
Please provide us with the information below, and we'll contact you as soon as possible.
  • HiPure Bacterial RNA Kit
Leave A Message