This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern Blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.
| CAT NO | PRODUCT NAME | SIZE | PRICE |
|---|---|---|---|
| D311302 | HiPure Tissue&Blood DNA Midi Kit | 20 preps | $224.00 |
| D311303 | HiPure Tissue&Blood DNA Midi Kit | 100 preps | $995.00 |
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern Blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 2ml blood and 200mg tissue using Midi column |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Midi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount | 0.2-2 ml |
| Elution volume | ≥300μl |
| Time per run | ≤80 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D311302 | D311303 |
| Purification Times | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml Collection Tubes | 40 | 200 |
| Buffer ATL | 50 ml | 250 ml |
|
Buffer AL |
50 ml | 250 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Buffer GW2* | 12 ml | 50 ml |
| RNase A | 20 mg | 90 mg |
| Proteinase K | 100 mg | 440 mg |
| Protease Dissolve Buffer | 10 ml | 30 ml |
| Buffer AE | 20 ml | 120 ml |
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
| Name | CAT NO |
Sample amount |
Leukocyte protocol* |
Colum type |
Elutio volume |
Average yield |
Time per run |
| D3111 | 10-250μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes | |
| D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes | |
| D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes | |
| D3117 | 1-200μl | 1ml | 96 well plate |
|
3-8μg/200μl |
|
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction