| CAT NO | PRODUCT NAME | SIZE | PRICE |
|---|---|---|---|
| R418502 | HiPure Stool RNA Kit | 50 preps | $298.00 |
| R418503 | HiPure Stool RNA Kit | 250 preps | $1,343.00 |
Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 100-150mg stool sample |
| Applications | RT-PCR, Northern hybridization and other experiments |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Stool |
| Sample amount | 100-200 mg |
| Elution volume | ≥30μl |
| Time per run |
≤50 minutes |
| Liquid carrying volume per column |
100µg |
| Binding yield of column | 800µl |
TheHiPure silica gel column uses a high binding ability glass fiber filtermembrane as the substrate. Under the condition of high concentration ofionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), thefilter membrane can adsorb nucleic acid through hydrogen bonding andelectrostatic and other physical factors, while protein or other impurities arenot adsorbed and removed. The filter membrane that has adsorbed nucleic acidsis washed to remove proteins and salts. Finally, low salt buffer solution (suchas Buffer TE) or water can be used to wash out the nucleic acids adsorbed onthe filter membrane. The obtained nucleic acid has high purity and can bedirectly used in various downstream experiments.
The stoolsamples are homogenized in the lysis solution, further lysed in ahigh-temperature water bath, and RNA is released into the lysis solution.Chloroform extraction removes genomic DNA and impurities, transfers thesupernatant to an alcohol free binding solution, purifies RNA through a column,and finally elutes RNA with RNase Free Water. The purified RNA can be directlyused for experiments such as PCR, Southern hybridization, and enzyme digestion.
Advantages
Kit Contents
| Contents | R418502 | R418503 |
| Purification Times | 50 Preps |
250 Preps |
| HiPure RNA Mini Columns | 50 | 250 |
|
2ml Collection Tubes |
50 |
250 |
|
Glass Beads (0.1~0.6mm) |
30 g |
150 g |
| Buffer SPL | 30 ml | 140 ml |
|
Buffer PHC |
30 ml |
140 ml |
|
Buffer GRP |
60 ml |
250 ml |
|
Buffer RW1 |
50 ml |
250 ml |
|
Buffer RW2 * |
20 ml |
2 x 50 ml |
|
RNase Free Water |
15 ml |
30 ml |
Storage and Stability
The kitcomponents can be stored at room temperature (15–25°C) and are stable for 18months under these conditions. At low temperatures, Buffer SPL may formprecipitates, dissolve it by 55°C water bath. After receiving the product,Buffer PHC should be stored at 2-8°C.
