banner
  • R661101-MagPure Blood RNA LQ Kit.png

R6611

CAT NO PRODUCT NAME SIZE PRICE
R661101 MagPure Blood RNA Kit 48 preps $286.00
R661102 MagPure Blood RNA Kit 96 preps $525.00
R661103 MagPure Blood RNA Kit 480 preps $2,100.00
RELATED TAGS: Clinical sample
Introduction

This product supplies a simple and rapid extraction of total RNA from Blood, buffy coat, bone marrow, cell suspension and other body fluids. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on.

Details

Specifications

Features Specifications
Main Functions Isolation total RNA from 300μl whole blood, bone marrow, buffy coat
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Whole blood,  buffy coat, bone marrow, cultured cells, lymphocytes, body fluid
Sample amount 5x106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow
Yield 1-30μg

Principle

The Kit can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5x106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.

Advantages

  • High purity - OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - extraction without manual participation, saving time and effort
  • High yield - nucleic acid recovery up to 95%
  • General - samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.

Kit Contents

Contents R661101 R661102 R661103
Purification Times 48 Preps 96 Preps
480 preps
MagPure RNA Particles 1.7 ml 3.5 ml
18 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
5 ml
15 ml
DNase I 600 μl 2 x 600 μl
10 x 600 µl
DNase Buffer
30 ml
40 ml
200 ml
Buffer MLB
40 ml
80 ml
350 ml
Buffer MCB* 15 ml 30 ml
150 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
15 ml
120 ml

Storage and Stability

MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide
Articles
Related Products
Leave A Message
Please provide us with the information below, and we'll contact you as soon as possible.
  • MagPure Blood RNA Kit
Leave A Message