Specifications
Features
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Specifications
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Main Functions
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Isolation total RNA from tissue, cell
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Applications
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RT-PCR, cDNA synthesis, second generation sequencing
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Adaptive instrument
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Nucleic acid extractor, pipetting workstation
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Sample type
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Tissues, cells, lymphocytes and other clinical sample
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Sample amount
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Cells grown insuspension:≤1 x 107
Animal tissue: ≤20mg
Plant tissue: ≤100mg
Whole blood: ≤1.5ml
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Yield
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2-50μg
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Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
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High purity - OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
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Economy - less than 50% of the price of Qiagen and other imported products
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Automatic - extraction without manual participation, saving time and effort
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Universal - suitable for various clinical samples
Kit Contents
Contents
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IVD3020
|
Purification Times
|
200 Preps
|
MagPure RNA Particles
|
7 ml
|
DNase I
|
4 x 600 µl
|
DNase Buffer
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80 ml
|
RTL Lysis Buffer
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150 ml
|
Buffer MCB*
|
75 ml
|
Buffer MW1*
|
110 ml
|
Buffer MW2*
|
50 ml
|
RNase Free Water
|
60 ml
|
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.