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IVD3020

CAT NO PRODUCT NAME SIZE PRICE
IVD3020 MagPure Universal RNA Kit 200 preps $895.00
RELATED TAGS: Clinical sample
Introduction

This kit is suitable for high-throughput extraction of total RNA from cultured cells, cell suspensions, animal tissues, conventional fungi and plant tissues. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 60 minutes. The obtained RNA can be directly used in RT-PCR, fluorescence quantification, second-generation sequencing and viral RNA detection and so on. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 hole oscillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Details

Specifications

Features Specifications
Main Functions Isolation total RNA from tissue, cell
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type Tissues, cells, lymphocytes and other clinical sample
Sample amount

Cells grown insuspension:1 x 107

Animal tissue: 20mg

Plant tissue: 100mg

Whole blood: 1.5ml

Yield 2-50μg

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.

Advantages

  • High purity - OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - extraction without manual participation, saving time and effort
  • Universal - suitable for various clinical samples

Kit Contents

Contents IVD3020
Purification Times 200 Preps
MagPure RNA Particles
7 ml
DNase I
4 x 600 µl
DNase Buffer
80 ml
RTL Lysis Buffer
150 ml
Buffer MCB*
75 ml
Buffer MW1* 110 ml
Buffer MW2*
50 ml
RNase Free Water
60 ml

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide
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  • MagPure Universal RNA Kit
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