Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column)
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Applications
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RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
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Purification method
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Mini spin column
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Purification technology
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Silica technology, DNA filtration technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
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Sample amount
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Cells:≤1 x 107
Animal tissue sample: 1-20 mg
Plant tissue: 50-150 mg
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Yield
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2-100μg
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Elution volume
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≥50μl
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Time per run
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≤25 minutes（1-24 samples）
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Liquid carrying volume per column
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800µl
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Binding yield of column
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100µg
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Principle
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Advantages
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Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
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High quality - high purity total RNA can be directly used in various sensitive downstream applications
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Fast - several samples can be extracted in 25 minutes by column method
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Safe - no phenol chloroform extraction required
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Sensitive - RNA can be recovered at the level of PG
Kit Contents
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Contents
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R411102
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D411103
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Purification Times
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50 Preps
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250 Preps
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HiPure DNA Mini Columns
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50
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250
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HiPure RNA Mini Columns
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50
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250
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2ml Collection Tubes
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100
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2 x 250
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Buffer RLC
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50 ml
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200 ml
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Buffer RW1
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50 ml
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200 ml
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Buffer RW2*
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12 ml
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2 x 50 ml
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RNase Free Water
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10 ml
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30 ml
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Storage and Stability
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
