This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
CAT NO | PRODUCT NAME | SIZE | PRICE |
---|---|---|---|
D314602 | HiPure Bacterial DNA Kit | 50 preps | $149.00 |
D314603 | HiPureBacterialDNA Kit | 250 preps | $646.00 |
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
Specifications
Features | Specifications |
Main Functions | Isolation bacterial DNA from cultures, food and other samples |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Culture medium, swab, parasitic blood, tissue,sputum, etc. |
Sample amount |
Bacterial culture medium: 0.5-2 ml
Tissue samples: 50-100 mg
Whole blood / cell suspension: 0.5-1 ml
Viscous secretion: 0.1-1 g |
Elution volume | ≥30μl |
Time per run | ≤40 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.
Kit Contents
Contents | D314602 | D314603 |
Purification Times | 50 Preps |
250 Preps |
Hipure DNA Mini Columns I | 50 |
2 x 125 |
2ml Collection Tubes | 50 |
2 x 125 |
Glass Beads (0.1~0.2mm) |
20 g |
100 g |
Buffer P1 |
20 ml |
100 ml |
Buffer DL | 15 ml |
80 ml |
Buffer GW1 |
22 ml |
88 ml |
Buffer GW2 |
12 ml |
50 ml |
Lysozyme | 60 mg |
300 mg |
Proteinase K | 24 mg |
120 mg |
Protease Dissolve Buffer | 5 ml |
15 ml |
Buffer AE |
15 ml |
60 ml |
Storage and Stability
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.