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D6323B

CAT NO PRODUCT NAME SIZE PRICE
D632301B MagPure FFPE DNA Kit 48 preps $199.00
D632302B
MagPure FFPE DNA Kit
96 preps
$373.00
RELATED TAGS: Cancer Research
Introduction

This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from FFPE using high bind beads
Applications RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Large quantities of solids
Sample amount Appropriate
Elution volume ≥50μl
Time per run 30 - 120 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High yield - most optimal process, recovery up to 90%
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Various operations - manual single tube/high-throughput or workstation
  • High purity - OD 260/280 : 1.7-1.9, OD 260/230 :1.5-2.0

Kit Contents

Contents D632301B D632302B
Purification Times 48 Preps
96 Preps
MagBind Particles 1.1 ml
2 x 1.1 ml
RNase A 10 mg
20 mg
Proteinase K
24 mg
48 mg
Protease Dissolve Buffer
3 ml
6 ml
Buffer DPS 60 ml
100 ml
Buffer ATL 15 ml
30 ml
Buffer AL 15 ml
30 ml
Buffer BD* 6 ml
15 ml
Buffer BXW1*
13 ml
44 ml
Elution Buffer 15 ml
30 ml

Storage and Stability

RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data


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  • MagPure FFPE DNA Kit
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