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  • IVD3101Total Nucleic Acid Extration.png

IVD3101

CAT NO PRODUCT NAME SIZE PRICE
IVD3101 MagPure DNA Micro Kit 200 preps $696.00
RELATED TAGS: Forensic Detecting
Introduction

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
Applications Second generation sequencing, PCR, real timePCR, etc.
Purification method
Monodisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount Body fluid : 10 - 200μl, Tissue : 10mg
Yield 10ng - 15μg
Elution volume 80 -100μl
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High binding force - suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
  • High purity - carboxyl magnetic beads with weak surface adsorption, getting higher purity
  • General - can be used for various clinical samples

Kit Contents

Contents IVD3101
Purification Times 200
MagBind Particles 4.5 ml
Proteinase K 90 mg
Protease Dissolve Buffer 10 ml
Buffer ATL 60 ml
Buffer AL 60 ml
Buffer BD* 20 ml
Buffer BXW1*
110 ml
Elution Buffer 30 ml

Storage and Stability

Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide
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