| CAT NO | PRODUCT NAME | SIZE | PRICE |
|---|---|---|---|
| D311102 | HiPure Blood DNA Mini Kit | 50 preps | $99.00 |
| D311102 | HiPure Blood DNA Mini Kit | 250 preps | $472.00 |
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 200ul Whole Blood |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells |
| Sample amount | 10-250ul |
| Elution volume | ≥20μl |
| Time per run | ≤30 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D311102 | D311103 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 2 x 125 |
| 2ml Collection Tubes | 100 | 5 x 100 |
| Buffer AL | 15 ml | 60 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer GW2 | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 15 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2–8°Cupon arrival. However, short-term storage (up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components canbe stored at room temperature (15–25°C) and are stable for at least 18 monthsunder these conditions.
| Name | CAT NO |
Sample amount |
Leukocyte protocol* |
Colum type |
Elutio volume |
Average yield |
Time per run |
| D3111 | 10-250μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes | |
| D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes | |
| D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes | |
| D3117 | 1-200μl | 1ml | 96 well plate |
|
3-8μg/200μl |
|
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
