Specifications
Features
|
Specifications
|
Main Functions
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Isolation total RNA from 50mg plant using magnetic particles
|
Applications
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RT-PCR, cDNA synthesis, second generation sequencing
|
Purification method
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Polydisperse magnetic beads
|
Purification technology
|
Magnetic beads technology
|
Process method
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Manual or automatic
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Sample type
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Plant and fungus samples
|
Sample amount
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≤50mg
|
Yield
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2-100μg
|
Time per run
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≤60 minutes
|
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
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High quality - high purity total RNA can be directly used in various sensitive downstream applications
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Safe - no phenol chloroform extraction required
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Fast - several samples can be extracted in 60 minutes by column method
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Universal - two lysates suitable for most plant or fungal tissue samples
Kit Contents
Contents
|
R664101
|
R664102
|
R664103
|
Purification Times
|
48 Preps
|
96 Preps
|
480 Preps
|
MagPure RNA Particles
|
1.7 ml
|
3.5 ml
|
18 ml
|
DNase I
|
600 μl
|
2 x 600 μl
|
10 x 600 μl
|
DNase Buffer
|
30 ml
|
40 ml
|
200 ml
|
Buffer PRC1
|
40 ml
|
70 ml
|
350 ml
|
Buffer RL
|
40 ml
|
70 ml
|
350 ml
|
Buffer MCB*
|
18 ml
|
30 ml
|
150 ml
|
Buffer MW1*
|
22 ml
|
44 ml
|
220 ml
|
Buffer MW2*
|
20 ml
|
50 ml
|
2 x 100 ml
|
RNase Free Water
|
10 ml
|
15 ml
|
120 ml
|
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these
conditions.