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R4151

CAT NO PRODUCT NAME SIZE PRICE
R415102 HiPure Plant RNA Kit 50 preps $149.00
R415103 HiPure Plant RNA Kit 250 preps $622.00
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Introduction

The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features Specifications
Main Functions Isolation total RNA from <150 mg simple plant sample (including plant sample containing starch) without chloroform
Applications RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method Mini spin column
Purification technology Silica technology, DNA filtration technology
Process method Manual (centrifugation or vacuum)
Sample type Economic crops
Sample amount
150 mg
Elution volume 30μl
Time per run ≤25 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg

Principle

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.

Advantages

  • Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - several samples can be extracted in 25 minutes by column method
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be recovered at the level of PG
  • Broad spectrum - various types of plant samples can be processed by diversity of operating procedures

Kit Contents

Contents R415102 D415103
Purification Times 50 Preps 250 Preps
HiPure DNA Mini Column II
50
250
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 100 2 x 250
Buffer RLC
50 ml
200 ml
Buffer PRC1
50 ml
200 ml
Buffer RW1 50 ml 200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.

Purchase Guide
Articles
Related Products
  • MagPure Plant RNA Kit

    Isolation total RNA from 50mg plant using magnetic particles

  • HiPure Plant RNA Midi Kit

    Isolation total RNA from <1g simple plant sample (including plant sample containing starch) without chloroform

  • HiPure Total RNA Kit

    Isolation total RNA from 20mg tissue, 150 mg plant, 5 x 10^6 cell using one column

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  • HiPure Plant RNA Kit
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