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P1006

CAT NO PRODUCT NAME SIZE PRICE
P100601 HiPure Plasmid Plus 96 Kit 1 x 96 preps $164.00
P100602 HiPure Plasmid Plus 96 Kit 4 x 96 preps $497.00
P100603 HiPure Plasmid Plus 96 Kit 20 x 96 preps $2,238.00
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Introduction

The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features Specifications
Main Functions Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate
Applications Enzyme digestion, sequencing, PCR, labeling, etc.
Purification method 96 well plate
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Conventional plasmid, plasmid less than 30KB
Sample amount
1-1.5ml(x96
Yield 1-15µg/1ml
Elution volume 70μl
Time per run 60 minutes
Liquid carrying volume per column 800µl
Binding yield of column 70µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
  • Fast - it takes only 60 minutes to complete the isolation
  • High yield - up to 1mg plasmid can be binded in one column
  • Economy - high cost performance

Kit Contents

Contents P100601 P100602 P100603
Purification Times 1 x 96 Preps
4 x 96 Preps 20 x 96 Preps
RNase A 5 mg
20 mg 100 mg
Buffer P1 30 ml
120 ml 600 ml
Buffer P2
30 ml
120 ml
600 ml
Buffer P3
40 ml
180 ml
800 ml
Buffer PW1
100 ml
500 ml
2 x 1000 ml
Buffer PW2 50 ml
2 x 100 ml 4 x 200 ml
Elution Buffer 150 ml
60 ml 300 ml
Lysate Clear Plate 1 4 20
HiPure DNA Plate 1 4 20
1.6 ml Collection Plate
1
4
20
0.5ml Elute Plate
1
4
20

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37 to dissolve. After addition of RNase ABuffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide
Name CAT NO

Sample amount

Column type

Elutio volume

Yield

Time per run

Centrifuge requirements

HiPure Plasmid Mini Kit

P1001 1-5ml 1.5ml column 30-50μl

5–35µg/5ml

≤30 minutes small centrifuge

HiPure Plasmid Mini Kit II

P1002 5-15ml 1.5ml column 75-100μl

20-80µg/15ml

≤30 minutes small centrifuge

HiPure Plasmid Plus 96 Kit

P1006 1-1.5ml 96 well plate 70-150μl

1-15μg/1ml

≤60 minutes 96 well plate centrifuge

HiPure Fastfilter Plasmid Midi Kit

P1013 30-50ml 15ml column 0.3-0.8ml

50–250µg/50ml

60 minutes
15ml centrifuge

HiPure Fastfilter Plasmid Maxi Kit

P1014
100-200ml
50ml column
0.5-1.5ml

0.4–1mg/200ml

60 minutes
50ml centrifuge
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