banner
  • IVD5412-F-96.png

IVD3020-F-96

CAT NO PRODUCT NAME SIZE PRICE
IVD3020-F-96 MagPure Universal RNA Precast Kit (96 channel machine) 96 preps $550.00
RELATED TAGS:
Introduction

This kit is suitable for high-throughput extraction of total RNA from cultured cells, cell suspensions, animal tissues, conventional fungi and plant tissues. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 60 minutes. The obtained RNA can be directly used in RT-PCR, fluorescence quantification, second-generation sequencing and viral RNA detection and so on. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 hole oscillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.

Details

Specifications

Features Specifications
Main Functions Isolation total RNA from tissue, cell
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type Tissues, cells, lymphocytes and other clinical sample
Sample amount

Cells grown insuspension:1 x 107

Animal tissue: 20mg

Plant tissue: 100mg

Whole blood: 1.5ml

Yield 2-50μg

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.

Advantages

  • High purity - OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - extraction without manual participation, saving time and effort
  • Universal - suitable for various clinical samples

Kit Contents

Cat.No Reagent
IVD3020-F-96
DNase Buffer
60 ml
DNase I

2 x 600 μl
RTL Lysis Buffer
80 ml
Buffer MCB
18 ml
96-Tip
1
Sample plate (DW Plate) 500μl Buffer MCB
1
Wash 1 Plate (DW Plate)

700μl Buffer MW1

30μl MagPure RNA Partilces

1
DNase Plate
Empty

Wash 2 Plate (DW Plate) 700μl Buffer MW1
1
Wash 3 Plate (DW Plate) 900μl Buffer MW2
1
Elution plate (DW Plate) 80μl RNase Free Water
1


Storage and Stability

DNase I should be shipped with ice pack and stored at -20°C after arrival. However, short-term storage (up to 1 week) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15–25°C) for 18 months.

Purchase Guide
Articles
Related Products
Leave A Message
Please provide us with the information below, and we'll contact you as soon as possible.
  • MagPure Universal RNA Precast Kit (96 channel machine)
Leave A Message