Specifications
Features
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Specifications
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Main Functions
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Isolation total DNA from 50mg simple plant using 96 well bind plate
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Applications
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PCR, SSR, AFLP, RAPD and southern blot, etc.
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Purification method
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96 well plate
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Economic plant samples
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Sample amount
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Fresh / frozen plant samples: ≤50mg
Dried plant / seed samples: ≤15mg
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Elution volume
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≥75μl
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Time per run
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30-50 minutes
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Liquid carrying volume per column
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800μl
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Binding yield of column
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100μg
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Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
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High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
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High throughput - 96 samples can be processed simultaneously by 96 well plate
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High permeability - using composite membrane technology, no hole plugging
Kit Contents
Contents
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D316702
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D316703
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Purification Times
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4 x 96 Preps
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20 x 96 Preps
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RNase Solution
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5 ml
|
22 ml
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Elution Buffer
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120 ml
|
500 ml
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Buffer SPL
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200 ml
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2 x 500 ml
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Buffer PS
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100 ml
|
400 ml
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Buffer GW1
|
220 ml
|
5 x 220 ml
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Buffer GW2
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100 ml
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3 x 100 ml
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1.6ml Collection Plate
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4
|
20
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HiPure gDNA Plate
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4
|
20
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2.2ml Collection Plate(DW Plate)
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8
|
40
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0.8ml Collection Plate(DW Plate)
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4
|
20
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Sealing Film
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20
|
100
|
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.