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D3167

CAT NO PRODUCT NAME SIZE PRICE
D316702 HiPure SF Plant DNA 96 Kit 4 x 96preps $796.00
D316703 HiPure SF Plant DNA 96 Kit 20 x 96preps $3,729.00
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Introduction

This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from 50mg simple plant using 96 well bind plate
Applications PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method 96 well plate
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Economic plant samples
Sample amount

Fresh / frozen plant samples: 50mg

Dried plant / seed samples: 15mg

Elution volume ≥75μl
Time per run 30-50 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg

Principle

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
  • High throughput - 96 samples can be processed simultaneously by 96 well plate
  • High permeability - using composite membrane technology, no hole plugging

Kit Contents

Contents D316702 D316703
Purification Times 4 x 96 Preps 20 x 96 Preps
RNase Solution
5 ml
22 ml
Elution Buffer
120 ml
500 ml
Buffer SPL 200 ml 2 x 500 ml
Buffer PS 100 ml 400 ml
Buffer GW1
220 ml
5 x 220 ml
Buffer GW2 100 ml 3 x 100 ml
1.6ml Collection Plate
4
20
HiPure gDNA Plate
4 20
2.2ml Collection Plate(DW Plate)
8 40
0.8ml Collection Plate(DW Plate)
4
20
Sealing Film
20
100

Storage and Stability

RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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  • HiPure SF Plant DNA 96 Kit
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