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D3117

CAT NO PRODUCT NAME SIZE PRICE
D311701 HiPure Tissue&Blood DNA 96 Kit 1 x 96 Preps $298.00
D311702 HiPure Tissue&Blood DNA 96 Kit 4 x 96 Preps $995.00
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Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications PCR, southern bolt and virus detection, etc
Purification method 96 well plate
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  • High throughput - 96 samples can be processed simultaneously

Kit Contents

Contents D311701 D311702
Purification Times 1 x 96 4 x 96
HiPure gDNA Plate 1 4
96 well Plate (2.2ml) 1 4
1.6ml Collection Plate 1 4
0.5ml Collection Plate 1 4
Silicon Seal Tape 1 4
Seal Film 5 25
Buffer ATL 30 ml 100 ml
Buffer AL 30 ml 100 ml
Buffer DW1 60 ml 250 ml
Buffer GW2 50 ml 2 x 100 ml
Proteinase K 50 ml 200 ml
Protease Dissolve Buffer 5 ml 15 ml
Buffer AE 30 ml 120 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name CAT NO

Sample amount

Leukocyte

protocol*

Colum type

Elutio volume

Average yield

Time per run

HiPure Blood DNA Mini Kit

D3111 10-200μl 1ml 2ml column ≥20μl 5-9μg/200μl ≤30 minutes

HiPure Tissue&Blood DNA Midi Kit

D3113 0.2-2ml 10ml 1.5ml column ≥300μl 20-40μg/1m ≤80 minutes

HiPure Tissue&Blood DNA Maxi Kit

D3115 3 -10ml 10ml 15ml column ≥700μl 20-40μg/1m ≤90 minutes

HiPure Tissue&Blood DNA 96 Kit

D3117 1-200μl 1ml 96 well plate
3-8μg/200μl

*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

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