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BCS

CAT NO PRODUCT NAME SIZE PRICE
BCS-5 CleanSeq Beads 5 ml $99.00
BCS-50 CleanSeq Beads 50 ml $895.00
BCS-500 CleanSeq Beads 500 ml $6,962.00
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Introduction

A rapid, high performance dye-terminator removal process based on the paramagnetic bead technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performedmanually or fully automated for high throughput dye-terminator removal. Compared to similar systems, this product produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.

Details

Specifications

Features Specifications
Main Functions
Removal of free fluorescent dye from sequencingsolution (Replace Beckmen or agencourt CleanSeq)
Applications Automated sequences
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type DNA doped with fluorescentdye
Sample amount
5μl
Recovery 90%
Elution volume
25μl
Operation time
50 minutes

Principle

The CleanSeq method contains magnetic particles in an optimized binding buffer to selectively capture sequencing extension products. The protocol can be performed directly in the thermal cycling plate. Unincorporated dyes, nucleotides, salts and contaminants are removed using a simple washing procedure. The purification procedure is amenable to a variety of automation platforms since it requires no centrifugation or vacuum filtration.

Advantages

  • High recovery - up to 90%
  • High throughput - using magnetic beads purification technology
  • Simple - simplified operation process, combination - washing - elution

Kit Contents

Contents BCS-5 BCS-50 BCS-500
CleanSeq Beads 5 ml
50 ml 500 ml

Storage and Stability

CleanSeq Beads should be stored at 2-8°C upon arrival and is stable up to 6 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Mix CleanSeq Beads well before using. The reagent should appear homogenous and consistent in color.

DO NOT FREEZE.

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